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anti-RXRA antibody product blog

Posted on 2015-09-01 23:06:23 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; RXRA; anti-RXRA antibody;
The RXRA rxra (Catalog #MBS129323) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RXRA Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s RXRA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
WB: 1:200 - 1:2000
IHC: 1:100 - 1:200
IF: 1:50 - 1:100. Researchers should empirically determine the suitability of the RXRA rxra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The RXRA rxra product has the following accession number(s) (GI #133701) (NCBI Accession #P19793.1) (Uniprot Accession #P19793). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RXRA Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of MCF-7 cells, using RXRA antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB) RXRA.

Retinoid X receptor alpha (RXRA). Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5\'-AGGTCA-3\' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid. RXRA serves as a common heterodimeric partner for a number of nuclear receptors. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5\'-AGGTCA-3\' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. The RXRA/PPARA heterodimer is required for PPARA transcriptional activity on fatty acid oxidation genes such as ACOX1 and the P450 system genes. This antibody is a rabbit polyclonal antibody raised against the 350 AA of human RXRA C-terminal. RXRA is highly expressed in liver, also expressed in lung, kidney and heart. It specifically recognizes the 51kd human RXRA protein.

Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human RXRA
Calculated Molecular Weight: 51kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RXRA are readily searchable from our website. Different antibodies against the same target such as RXRA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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