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anti-SCARB2 antibody product blog

Posted on 2016-06-28 16:06:48 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; anti-SCARB2 antibody; SCARB2;
The SCARB2 scarb2 (Catalog #MBS9126347) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SCARB2 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s SCARB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the SCARB2 scarb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The SCARB2 scarb2 product has the following accession number(s) (GI #2498525) (NCBI Accession #Q14108.2) (Uniprot Accession #Q14108). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SCARB2 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using SCARB2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.)
Western Blot (WB) SCARB2.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat heart using SCARB2 Antibody at dilution of 1:100 (20x lens).)
Immunohistochemistry (IHC) SCARB2.

The protein encoded by this gene is a type III glycoprotein that is located primarily in limiting membranes of lysosomes and endosomes. Earlier studies in mice and rat suggested that this protein may participate in membrane transportation and the reorganization of endosomal/lysosomal compartment. The protein deficiency in mice was reported to impair cell membrane transport processes and cause pelvic junction obstruction, deafness, and peripheral neuropathy. Further studies in human showed that this protein is an ubiquitously expressed protein and that it is involved in the pathogenesis of HFMD (hand, foot, and mouth disease) caused by enterovirus-71 and possibly by coxsackievirus A16. Mutations in this gene caused an autosomal recessive progressive myoclonic epilepsy-4 (EPM4), also known as action myoclonus-renal failure syndrome (AMRF). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human SCARB2
Calculated Molecular Weight: 54kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SCARB2 are readily searchable from our website. Different antibodies against the same target such as SCARB2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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