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anti-SCF antibody product blog

Posted on 2015-10-16 10:47:10 by mybiosource_staff
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Tags: Antibody; SCF; Polyclonal Antibody; anti-SCF antibody;
The SCF kitlg (Catalog #MBS691239) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Anti-Rat SCF reacts with Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s SCF can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Neutr.
Western Blot: To detect rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat SCF is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Researchers should empirically determine the suitability of the SCF kitlg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The SCF kitlg product has the following accession number(s) (GI #33667068) (NCBI Accession #NP_068615.1) (Uniprot Accession #P21581). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. Recombinant rat SCF is an 18.4 kDa polypeptide containing 165 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.

SCF kitlg

Buffer: PBS; pH 7.2
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rat SCF(15 ng/ml), a concentration of 0.08-0.12 ug/ml of this antibody is required. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SCF are readily searchable from our website. Different antibodies against the same target such as SCF may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Adipose Tissue, Brain, Embryonic Tissue, Eye, Heart, Lung, Muscle, Ovary, Placenta, Prostate tissues are correlated with this protein. Anemia, Bone Marrow Diseases, Carcinoma, Cardiovascular Diseases, Heart Diseases, Inflammation, Myocardial Ischemia, Necrosis, Neoplasms, Germ Cell and Embryonal, Nervous System Diseases are some of the diseases may be linked to Goat Anti-Rat SCF.
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