anti-SERPINB5 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-SERPINB5 antibody; SERPINB5;
The SERPINB5 serpinb5 (Catalog #MBS127847) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SERPINB5 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s SERPINB5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the SERPINB5 serpinb5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SERPINB5 serpinb5 product has the following accession number(s) (GI #229462757) (NCBI Accession #P36952.2) (Uniprot Accession #P36952). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SERPINB5 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using SERPINB5 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.)
Maspin (SERPINB5) was discovered as a mammary tumor suppressor that is expressed in normal mammary epithelium but lost in most breast cancer cell lines (1). While maspin is related to the serpin family of serine protease inhibitors, it may not function as a protease inhibitor (2). It plays an essential role in embryonic development through critical roles in cell adhesion (3). While the precise mechanism of maspin signaling is unclear (4), the tumor suppressing activity of maspin has been attributed to its ability to inhibit cell invasion/metastasis (5,6) and angiogenesis (7), while promoting apoptosis (8). Nuclear translocation of active IKKalpha has been shown to repress maspin transcription and promote prostate cancer metastasis (9).
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human SERPINB5
Calculated Molecular Weight: 42kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SERPINB5 are readily searchable from our website. Different antibodies against the same target such as SERPINB5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Adenocarcinoma, Breast Neoplasms, Carcinoma, Colorectal Neoplasms, Inflammation, Lung Diseases, Lung Neoplasms, Neoplasm Invasiveness, Neoplasm Metastasis, Neoplasms, Experimental are some of the diseases may be linked to SERPINB5 Polyclonal Antibody.