anti-SETDB1 antibody product blog
Tags: Antibody; Polyclonal Antibody; SETDB1; anti-SETDB1 antibody;
The SETDB1 setdb1 (Catalog #MBS1496950) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SETDB1 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s SETDB1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).Recommended dilution: WB:1:200-1000
IHC:1:20-1:200. Researchers should empirically determine the suitability of the SETDB1 setdb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SETDB1 setdb1 product has the following accession number(s) (GI #224177467) (NCBI Accession #NP_001138887.1) (Uniprot Accession #Q15047). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SETDB1 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot
All lanes: SETDB1 antibody at 5ug/ml
Lane 1:rat adrenal gland tissue
Lane 2:293T whole cell lysate
Secondary
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 144,45 kDa
Observed band size: 143 kDa
)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney using MBS1496950 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta using MBS1496950 at dilution 1:100)
Histone methyltransferase that specifically trimethylates \'Lys-9\' of histone H3. H3 \'Lys-9\' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 \'Lys-9\' trimethylation is coordinated with DNA methylation. Probably forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone \'Lys-9\' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins.
Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugate: Non-conjugated. Immunogen: Recombinant human Histone-lysine N-methyltransferase SETDB1 protein(1-397aa)
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-46112 / sc-66884. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SETDB1 are readily searchable from our website. Different antibodies against the same target such as SETDB1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.