anti-SHC1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-SHC1 antibody; SHC1; Shc;
The SHC1 shc1 (Catalog #MBS615935) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Shc, phosphorylated (Tyr317) (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Shc can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Dilution: Western Blotting: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Researchers should empirically determine the suitability of the SHC1 shc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SHC1 shc1 product has the following accession number(s) (GI #32261324) (NCBI Accession #NP_003020.2) (Uniprot Accession #P29353). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Shc, phosphorylated (Tyr317) (SHC1, Src homology and collagen, Src homology 2, FLJ26504, p52SHC, p66SHC, SH2 domain protein C1, SHC, SHCA, SHC transforming protein 1) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from EGF (100 ng/ml) treated A431 and HepG2 cells after 24 hours’ starvation in serum-free medium, using MBS615935 Antibody (upper) and Shc antibody (lower).)
Shc possesses SH2 and PTB domains and serves a scaffolding function in signaling for a variety of receptor tyrosine kinases. Shc exists in p46, p52 and p66 isoforms, which are produced by using alternative translation initiation sites or a differentially spliced message (1-3). In response to extracellular signals, the SH2 and PTB domains of Shc interact with the activated receptors, leading to phosphorylation of Shc on three different tyrosine residues: Tyr239, Tyr240 and Tyr317 (4-6). GRB2/Sos binds to Shc phosphorylated at these sites, activating the Ras/Raf/MAPK pathway (4). Both Shc expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T cell development (7).
Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr317 of human Shc. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SHC1 are readily searchable from our website. Different antibodies against the same target such as SHC1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.