|The SHH shh (Catalog #MBS9403539) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SHH Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s SHH can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC).
Immunohistochemistry: 1:50-1:200. Researchers should empirically determine the suitability of the SHH shh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SHH shh product has the following accession number(s) (GI #4506939) (NCBI Accession #NP_000184.1) (Uniprot Accession #Q15465). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SHH Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using at dilution 1/70.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using at dilution 1/70.)
This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.
Immunogen Type: Peptide
Immunogen Description: Synthetic peptide corresponding to residues near the C terminal of human Sonic hedgehog. Target Name: SHH. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SHH are readily searchable from our website. Different antibodies against the same target such as SHH may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.