anti-SIRT1 antibody product blog
Tags: Antibody; Polyclonal Antibody; SirT1; anti-SIRT1 antibody; SIRT1 / Sirtuin 1;
The SIRT1 sirt1 (Catalog #MBS243883) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Polyclonal to Human SIRT1 / Sirtuin 1 reacts with Gibbon, Gorilla, Human, MonkeyPredicted Reactivity: Mouse (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. MyBioSource\'s SIRT1 / Sirtuin 1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA).
ELISA (1:64000), IHC-P (3.75 ug/ml), WB (0.05 - 0.2 ug/ml)
Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 120kD band observed in lysates of cell line Jurkat (calculated MW of 81.7kD according to NP_036370.2). The observed molecular weight corresponds to earlier findings in lit.. Researchers should empirically determine the suitability of the SIRT1 sirt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SIRT1 sirt1 product has the following accession number(s) (GI #7657575) (NCBI Accession #NP_036370.2) (Uniprot Accession #Q96EB6). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat Polyclonal to Human SIRT1 / Sirtuin 1 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-SIRT1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.)
Western Blot (WB) (SIRT1 antibody (0.05 ug/ml) staining of Jurkat lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
The SIRT family of genes are highly conserved from prokaryotes to eukaryotes and are involved in diverse processes including transcriptional regulation, cell cycle progression, DNA damage repair and aging. Sirtuins utilize NAD+ as a substrate to catalyze deacetylation of specific acetylated protein and histones. SIRT1 has been shown to be induced by caloric restriction (CR) in several tissues and SIRT1 mediated deacetylase activity attenuates B-amyloid content in the brain during CR.
Target Species: Human
Immunogen Description: Synthetic peptide C-EKPQEVQTSRNVES from an internal region of human SIRT1 (NP_036370.2; NP_001135970.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mouse (93%); Rat (86%). Immunogen Type: Synthetic peptide
Immunogen: SIRT1 / Sirtuin 1 antibody was raised against synthetic peptide C-EKPQEVQTSRNVES from an internal region of human SIRT1 (NP_036370.2; NP_001135970.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%); Mouse (93%); Rat (86%).
Antigen Modification: Internal. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SIRT1 are readily searchable from our website. Different antibodies against the same target such as SIRT1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.