anti-Sirt3 antibody product blog
Tags: Antibody; Polyclonal Antibody; SIRT3; anti-SIRT3 antibody; M Sirt3;
The Sirt3 sirt3 (Catalog #MBS9210535) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The M Sirt3 Antibody (C-term) reacts with Mouse, hamster and may cross-react with other species as described in the data sheet. MyBioSource\'s M Sirt3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P).WB~~1:2000
IHC-P~~1:50~100. Researchers should empirically determine the suitability of the Sirt3 sirt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Sirt3 sirt3 product has the following accession number(s) (GI #188219522) (NCBI Accession #NP_001120823.1) (Uniprot Accession #Q8R104). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the M Sirt3 Antibody (C-term) with the following immunoassay(s):
Western Blot (Anti-Sirt3 Antibody (C-term) at 1:64000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (Anti-Sirt3 Antibody (C-term) at 1:2000 dilution + CHO whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot ( Anti-Mouse Sirt3 Antibody (C-term) at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
Immunohistochemistry (IHC) (Sirt3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Sirt3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.
Function: NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, PDHA1, SOD2, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
Cellular Location: Isoform L: Mitochondrion matrix.
Tissue Location: Strongly expressed in liver and kidney. Weakly expressed in lung.