anti-SIRT7 antibody product blog
Tags: Antibody; Polyclonal Antibody; SIRT7; anti-SIRT7 antibody;
The SIRT7 sirt7 (Catalog #MBS7051428) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIRT7 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s SIRT7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF).IHC: 1: 20-1: 200
IF: 1: 50-1: 200. Researchers should empirically determine the suitability of the SIRT7 sirt7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SIRT7 sirt7 product has the following accession number(s) (GI #7706712) (NCBI Accession #NP_057622.1) (Uniprot Accession #Q9NRC8). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SIRT7 Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7051428 at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells using MBS7051428 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
NAD-dependent protein deacetylase that specifically mediates deacetylation of histone H3 at \'Lys-18\' (H3K18Ac). In contrast to other histone deacetylases, displays selectivity for a single histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors. SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. These data suggest that SIRT7 may play a key role in oncogenic transformation by suppresses expression of tumor suppressor genes by locus-specific deacetylation of H3K18Ac at promoter regions. Also required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis: promotes the association of RNA polymerase I with the rDNA promoter region and coding region. Stimulates transcription activity of the RNA polymerase I complex. May also deacetylate p53/TP53 and promotes cell survival, however such data need additional confirmation.
Immunogen: Recombinant Human NAD-dependent protein deacetylase sirtuin-7 protein (242-379AA). Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugation: Non-conjugated
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-66281 / sc-66283. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SIRT7 are readily searchable from our website. Different antibodies against the same target such as SIRT7 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.