|The Slc12a1 slc12a1 (Catalog #MBS805560) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NKCC2 Antibody reacts with Human (weak), Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NKCC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoblot (IB).
1:1000 (WB). Researchers should empirically determine the suitability of the Slc12a1 slc12a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Slc12a1 slc12a1 product has the following accession number(s) (GI #162287159) (NCBI Accession #NP_062007) (Uniprot Accession #Q5PQV1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NKCC2 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Rat kidney tissue lysates showing detection of NKCC2 protein using Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401). Primary Antibody: Rabbit Anti-NKCC2 Polyclonal Antibody (SPC-401) at 1:1000.)
Background Info: Detects ~160kDa
Scientific Background: NKCC2, a kidney-specific member of the cation chloride co-transporter family SLC12, facilitates transport of sodium, potassium, and chloride ions from the lumen of the renal thick ascending limb into the cell (1, 2). Transport of sodium dilutes the luminal fluid, decreasing its osmolality creating an osmotic driving force for water reabsorption in the connecting tubule and cortical collecting duct under the influence of the hormone vasopressin. NKCC2 is found on the apical surface of the thick ascending limb of the loop of Henle and is blocked by loop diuretics such as furosemide (1).
Certificate of Analysis: 1 ug/mL of SPC-401 was sufficient for detection of NKCC2 in 10ug of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
Conjugate: Unconjugated. Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Slc12a1 are readily searchable from our website. Different antibodies against the same target such as Slc12a1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Kidney tissues are correlated with this protein. The following patways have been known to be associated with this gene.