anti-SLC12A2 antibody product blog
Tags: Antibody; Polyclonal Antibody; SLC12A2; anti-SLC12A2 antibody; NKCC1;
The SLC12A2 slc12a2 (Catalog #MBS176766) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NKCC1 antibody reacts with Reacts with: Human, RatPredicted to work with: Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s NKCC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Frozen Section.
Western Blot:
Concentration: 0.1-0.5 ug/ml
Tested Species: Hu
Predicted Species: Ms, Rat
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1 ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: By Heat
Immunohistochemistry (Frozen Section):
Concentration: 0.5-1 ug/ml
Tested Species: Rat
Predicted Species: Hu, Ms
Tested Species: In-House tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins, is required for the staining of formalin/paraffin sections.
Other applications have not been tested. Optimal dilutions should be determined by end user. Researchers should empirically determine the suitability of the SLC12A2 slc12a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SLC12A2 slc12a2 product has the following accession number(s) (GI #1709292) (NCBI Accession #P55011.1) (Uniprot Accession #P55011). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Solute carrier family 12(sodium/potassium/chloride transporters), member 2, also known as NKCC1, is widely distributed throughout the body, especially in organs that secrete fluids, called exocrine glands. By fluorescence in situ hybridization, this gene is mapped to chromosome 5q23.3. The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene.
Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SLC12A2 (223-241aa RIDHYRHTAAQLGEKLLRP), identical to the related mouse and rat sequences. Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SLC12A2 are readily searchable from our website. Different antibodies against the same target such as SLC12A2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.