anti-SLC12A3 antibody product blog
Tags: Antibody; Polyclonal Antibody; SLC12A3; anti-SLC12A3 antibody; NCC;
The SLC12A3 slc12a3 (Catalog #MBS806005) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCC Antibody: ATTO 488 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NCC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF).1:1000 (WB). Researchers should empirically determine the suitability of the SLC12A3 slc12a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SLC12A3 slc12a3 product has the following accession number(s) (GI #186910315) (NCBI Accession #NP_000330) (Uniprot Accession #P55018). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NCC Antibody: ATTO 488 with the following immunoassay(s):
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:200. Secondary Antibody: FITC Goat Anti-Rabbit (green).)
Western Blot (WB) (Western blot analysis of Rat tissue lysates showing detection of NCC protein using Rabbit Anti-NCC Polyclonal Antibody (SPC-402). Primary Antibody: Rabbit Anti-NCC Polyclonal Antibody (SPC-402) at 1:1000.)
Background Info: Detects ~160kDa
Scientific Background: NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
Conjugate: ATTO 488. Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SLC12A3 are readily searchable from our website. Different antibodies against the same target such as SLC12A3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.