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anti-Slc9a3 antibody product blog

Posted on 2015-12-05 22:25:25 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; NHE3; SLC9A3; anti-Slc9a3 antibody;
The Slc9a3 slc9a3 (Catalog #MBS806321) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NHE3 Antibody: ATTO 565 reacts with Rat, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s NHE3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).
1:1000 (WB). Researchers should empirically determine the suitability of the Slc9a3 slc9a3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Slc9a3 slc9a3 product has the following accession number(s) (GI #6981562) (NCBI Accession #NP_036786) (Uniprot Accession #P26433). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NHE3 Antibody: ATTO 565 with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Rat kidney tissue lysates showing detection of NHE3 protein using Rabbit Anti-NHE3 Polyclonal Antibody (SPC-400). Primary Antibody: Rabbit Anti-NHE3 Polyclonal Antibody (SPC-400) at 1:1000.)
Western Blot (WB) Slc9a3.

Background Info: Detects ~84kDa

Scientific Background: Sodium-hydrogen exchanger 3 (NHE3; Slc9a3) is an epithelial transport protein that carries out 1:1 exchange of Na+ and H+ across the plasma membrane. It is apically located in the proximal tubule of the kidney, the thick ascending limb of the kidney, and in small intestine (1). NHE3 is phosphorylated and regulated by multiple kinases including PKA, SGK1 and CK2. It can be phosphorylated by calyculin A, and dephosphorylated by PP1 catalytic subunit in vitro (2).

Conjugate: ATTO 565. Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Slc9a3 are readily searchable from our website. Different antibodies against the same target such as Slc9a3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Slc9a3 also interacts with the following gene(s): Cftr. CYSTIC FIBROSIS, MODIFIER OF, 1, Cardiovascular Abnormalities, Fibrosis, Heart Failure, Hyperglycemia, Hypertension, Inflammation, Intestinal Obstruction, Kidney Diseases, Seizures are some of the diseases may be linked to NHE3 Antibody: ATTO 565.
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