anti-SMAD2 antibody product blog
Tags: Antibody; Polyclonal Antibody; SMAD2; anti-SMAD2 antibody;
The SMAD2 smad2 (Catalog #MBS617171) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Smad2, phosphorylated (Ser245/250/255) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Smad2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Suitable for use in ELSA and Western Blot.
Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the SMAD2 smad2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SMAD2 smad2 product has the following accession number(s) (GI #2967646) (Uniprot Accession #Q15796). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Smad2, phosphorylated (Ser245/250/255) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from HeLa cells, mock transfected or transfected with Smad2 or Smad3 and treated with TPA (200 nM) or TGF-beta (100 ng/ml) for 30 minutes, using MBS617171 Smad2 Phosphorylated (Ser245/250/255).)
Western Blot (WB) (Western blot analysis of extracts from 293 and HeLa cells, untreated or TPA-treated (200 nM for 30 minutes) using MBS617171 Smad2 Phosphorylated (Ser245/250/255) (upper) or Smad2 (lower).)
Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-b signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Briefly, activated type I receptors associatewith specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8). Oncogenic Ras antagonizes TGF-b signaling and inhibits the nuclear accumulation of Smad2 and Smad3, which may be explained through MAP kinase dependent phosphorylation of these Smads (9). Cell stimulation with EGF leads to phosphorylation of Smad2 at a cluster of serine-proline sites within its linker region, including Ser245, 250, and 255 (9).
Immunogen: Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding serines 245/250/255 of Smad2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SMAD2 are readily searchable from our website. Different antibodies against the same target such as SMAD2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.