anti-SMAD4 antibody product blog
Tags: Antibody; SMAD4; anti-SMAD4 antibody; Polyclonal Antibody;
The SMAD4 smad4 (Catalog #MBS631176) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMAD4, CT (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP Homolog 4, Deletion Target In Pancreatic Carcinoma 4, MAD Homolog 4, SMAD Family Member 4, hSMAD4, DPC4, MADH4) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s SMAD4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF).Suitable for use in ELISA, Western Blot, Immunohistochemistry, Flow Cytometry and Immunofluorescence.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunohistochemistry: 1:50-1:100
Flow cytometry: 1:10~1:50
Immunofluorescence: 1:10~1:50. Researchers should empirically determine the suitability of the SMAD4 smad4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SMAD4 smad4 product has the following accession number(s) (GI #13603414) (Uniprot Accession #Q9BYG6). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SMAD4, CT (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP Homolog 4, Deletion Target In Pancreatic Carcinoma 4, MAD Homolog 4, SMAD Family Member 4, hSMAD4, DPC4, MADH4) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of SMAD4 (arrow) using rabbit polyclonal SMAD4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SMAD4 gene (Lane 2).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human skeletal muscle reacted with SMAD4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunofluorescence (IF) (Fluorescent confocal image of Hela cell stained with SMAD4 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD4 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SMAD4 immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
Flow Cytometry (FC/FACS) (SMAD4 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SMAD4 is the common SMAD (co-SMAD)mediator of signal transduction by TGF-beta (transforming growth factor). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.