anti-SMN1 antibody product blog
Tags: Antibody; Polyclonal Antibody; SMN1/SMN2; SMN1; anti-SMN1 antibody;
The SMN1 smn1 (Catalog #MBS6003424) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The SMN1/SMN2 (Survival Motor Neuron Protein, Component of Gems 1, Gemin-1, SMN, SMNT, SMNC) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s SMN1/SMN2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:32,000
Western Blot: 0.3-1ug/ml, observed in human brain, cerebellum lysates on ~26kD bands. Researchers should empirically determine the suitability of the SMN1 smn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SMN1 smn1 product has the following accession number(s) (GI #13259512) (NCBI Accession #NP_075012.1) (Uniprot Accession #Q16637). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SMN1/SMN2 (Survival Motor Neuron Protein, Component of Gems 1, Gemin-1, SMN, SMNT, SMNC) with the following immunoassay(s):
Chemiluminescence ((1ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
SMN1/SMN2 is part of a 500kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. The telomeric and centromeric copies of this gene are nearly identical and encode the same protein. While mutations in the telomeric copy are associated with spinal muscular atrophy, mutations in this gene, the centromeric copy, do not lead to disease. This gene may be a modifier of disease caused by mutation in the telomeric copy. The critical sequence difference between the two genes is a single nucleotide in exon 7 which is thought to be an exon splice enhancer. It is thought that gene conversion events may involve the two genes, leading to varying copy numbers of each gene. The full length protein encoded by this gene localizes to both the cytoplasm and the nucleus. Within the nucleus, the protein localizes to subnuclear bodies called gems which are found near coiled bodies containing high concentrations of small ribonucleoproteins (snRNPs). This protein forms heteromeric complexes with proteins such as SIP1 and GEMIN4, and also interacts with several proteins known to be involved in the biogenesis of snRNPs, such as hnRNP U protein and the small nucleolar RNA binding protein.