anti-SMN1 antibody product blog
Tags: Antibody; Polyclonal Antibody; SMN1; anti-SMN1 antibody;
The SMN1 smn1 (Catalog #MBS7050135) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMN1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s SMN1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).WB: 1: 500-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200. Researchers should empirically determine the suitability of the SMN1 smn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SMN1 smn1 product has the following accession number(s) (GI #4507091) (NCBI Accession #NP_000335.1) (Uniprot Accession #Q16637). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SMN1 Antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot
Positive WB detected in: A549 whole cell lysate,Hela whole cell lysate
All lanes: SMN1 antibody at 2ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 32,29,31,28 KDa
Observed band size: 40,60 KDa
)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis tissue using MBS7050135 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of HepG2 cells using MBS7050135 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus. Ensures the correct splicing of U12 intron-containing genes that may be important for normal motor and proprioceptive neurons development. Also required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination. May also play a role in the metabolism of small nucleolar ribonucleoprotein (snoRNPs).
Immunogen: Recombinant Human Survival motor neuron protein protein (3-194AA). Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Conjugation: Non-conjugated
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-137200 / sc-15320 / sc-7804.