anti-SMO antibody product blog
Tags: Antibody; Polyclonal Antibody; SMO; anti-SMO antibody;
The SMO smo (Catalog #MBS7051048) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMO Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s SMO can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).WB: 1: 1000-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200. Researchers should empirically determine the suitability of the SMO smo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SMO smo product has the following accession number(s) (GI #5032099) (NCBI Accession #NP_005622.1) (Uniprot Accession #Q99835). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SMO Antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot
Positive WB detected in:Mouse liver tissue,Mouse kidney tissue
All lanes: SMO antibody at 2ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 87 kDa
Observed band size: 87 kDa
)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver tissue using MBS7051048 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of A549 cells using MBS7051048 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog\'s proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7 and GLI3 in the cilia.
Immunogen: Recombinant human Smoothened homolog protein (28-233AA). Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugation: Non-conjugated
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-6366 / sc-13943. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SMO are readily searchable from our website. Different antibodies against the same target such as SMO may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Bone, Bone Marrow, Brain, Embryonic Tissue, Eye, Heart, Lung, Muscle, Prostate, Skin tissues are correlated with this protein. Brain Neoplasms, Breast Neoplasms, Carcinoma, Basal Cell, Cell Transformation, Neoplastic, Disease Models, Animal, Lung Neoplasms, Neoplasm Metastasis, Neoplasms, Experimental, Neuroectodermal Tumors, Primitive, Skin Neoplasms are some of the diseases may be linked to SMO Antibody.