|The Smp_013060 smad1 (Catalog #MBS615510) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Smad1, phosphorylated (Ser463,465) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma) reacts with Human, Mouse, Rat, Xenopus and may cross-react with other species as described in the data sheet. MyBioSource\'s Smad1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
Suitable for use in Western Blot.
Dilution: Immunoblot: 0.5-2ug/ml detected phosphorylated Smad1 in RIPA lysates from P19 (mouse embryonal carcinoma) cells treated with BMP4. P19 cell lysate treated with BMP4 was resolved by electrophoresis, transferred to nitrocellulose and probed with phospho-Smad1 (Ser463/465) (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Note: A non-specific band was detected in P19 cell lysate, Mr 34-35kD. Researchers should empirically determine the suitability of the Smp_013060 smad1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Smp_013060 smad1 product has the following accession number(s) (GI #11464653) (Uniprot Accession #Q9GQN9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The Drosophila gene Mothers against dpp (Mad) and the C. elegans sma genes are implicated in the signaling pathway downstream of Ser/Thr kinase receptors required for signaling by the TGFb superfamily. Phosphorylation of Smad1, a human homolog of MAD is regulated and rapidly induced by bone morphogenic proteins (BMP) but not TGFb or activin. BMPs play a role in ectopic bone formation and inductive interactions during the early development of both vertebrates and invertebrates. In the mouse, deletion of the BMP4 gene leads to mesoderm formation and early embryonic lethality. Phosphorylation of MADR1 in a BMP dependent manner is functionally important in that it results in accumulation of Smad1 in the nucleus, indicative of Smad and Smad-related molecules as essential components of the Ser/Thr kinase receptor signaling pathway.
Immunogen: Synthetic peptide (CG-GSPHNPIS[pS]V[pS]-GG) corresponding to amino acids 455-465 of human Smad1 protein (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Smp_013060 are readily searchable from our website. Different antibodies against the same target such as Smp_013060 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.