Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Polyclonal Antibody Blog  rightarrow anti-Smp_013060 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • anti-C-Myc antibody blog
 • anti-ACACA antibody blog
 • anti-ARL1 antibody blog
 • anti-MZF1 antibody blog
 • anti-ApoB antibody blog
 • anti-Epcam antibody blog
 • anti-ZFP64 antibody blog
 • anti-APEX1 antibody blog
 • anti-AKT1 antibody blog
 • anti-PHB antibody blog
 • anti-cpg2 antibody blog
 • anti-BNIP1 antibody blog
 • anti-GUSB antibody blog
 • anti-NAA60 antibody blog
 • anti-ACE2 antibody blog
 • anti-TNFRSF1A antibody blog
 • anti-TIMP3 antibody blog
 • anti-GYS2 antibody blog
 • anti-ZNF433 antibody blog
 • anti-AKT2 antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • TST Antibody
 • Dengue Virus Subtype 1 Envelope 45kDa Recombinant Protein
 • Complement Component 4B (C4B) ELISA Kit
 • Tissue, Total Protein Adult Normal, Brain, Pons Lysate
 • ku86 ELISA Kit
 • African Horse Sickness Virus PCR Kit
 • Anti-Type II Collagen Antibody (CII) ELISA Kit
 • TMB Substrate for ELISA Substrate
 • KLHL23 Antibody
 • ZDHHC2 Antibody
 • CD48 Antibody
 • MNDA Antibody
 • Copine VIII (CPNE8) ELISA Kit
 • Angiotensin I Converting Enzyme (ACE) ELISA Kit
 downarrow more ...
separator

anti-Smp_013060 antibody product blog

separator
Posted on 2015-11-05 05:59:26 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Antibody; Smad1; Polyclonal Antibody; anti-Smp_013060 antibody; Smp_013060;
separator
The Smp_013060 smad1 (Catalog #MBS615510) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Smad1, phosphorylated (Ser463,465) (Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma) reacts with Human, Mouse, Rat, Xenopus and may cross-react with other species as described in the data sheet. MyBioSource\'s Smad1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
Suitable for use in Western Blot.
Dilution: Immunoblot: 0.5-2ug/ml detected phosphorylated Smad1 in RIPA lysates from P19 (mouse embryonal carcinoma) cells treated with BMP4. P19 cell lysate treated with BMP4 was resolved by electrophoresis, transferred to nitrocellulose and probed with phospho-Smad1 (Ser463/465) (1ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Note: A non-specific band was detected in P19 cell lysate, Mr 34-35kD. Researchers should empirically determine the suitability of the Smp_013060 smad1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Smp_013060 smad1 product has the following accession number(s) (GI #11464653) (Uniprot Accession #Q9GQN9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


The Drosophila gene Mothers against dpp (Mad) and the C. elegans sma genes are implicated in the signaling pathway downstream of Ser/Thr kinase receptors required for signaling by the TGFb superfamily. Phosphorylation of Smad1, a human homolog of MAD is regulated and rapidly induced by bone morphogenic proteins (BMP) but not TGFb or activin. BMPs play a role in ectopic bone formation and inductive interactions during the early development of both vertebrates and invertebrates. In the mouse, deletion of the BMP4 gene leads to mesoderm formation and early embryonic lethality. Phosphorylation of MADR1 in a BMP dependent manner is functionally important in that it results in accumulation of Smad1 in the nucleus, indicative of Smad and Smad-related molecules as essential components of the Ser/Thr kinase receptor signaling pathway.

Smp_013060 smad1

Immunogen: Synthetic peptide (CG-GSPHNPIS[pS]V[pS]-GG) corresponding to amino acids 455-465 of human Smad1 protein (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Smp_013060 are readily searchable from our website. Different antibodies against the same target such as Smp_013060 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions