anti-SMYD4 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-SMYD4 antibody; SMYD4;
The SMYD4 smyd4 (Catalog #MBS6003707) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SMYD4, CT (SMYD4, KIAA1936, SET and MYND domain-containing protein 4) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s SMYD4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100
Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the SMYD4 smyd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SMYD4 smyd4 product has the following accession number(s) (GI #156616308) (NCBI Accession #NP_443160.2) (Uniprot Accession #Q8IYR2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SMYD4, CT (SMYD4, KIAA1936, SET and MYND domain-containing protein 4) with the following immunoassay(s):
Western Blot (WB) (SMYD4 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the SMYD4 antibody detected the SMYD4 protein (arrow).)
Immunohistochemistry (IHC) (SMYD4 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SMYD4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (SMYD4 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
The specific function of the protein remains unknown.
Immunogen: SMYD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 548-576 amino acids from the C-terminal region of human SMYD4. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SMYD4 are readily searchable from our website. Different antibodies against the same target such as SMYD4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.