anti-SNAP-25 antibody product blog
Tags: Antibody; Polyclonal Antibody; SNAP-25; anti-SNAP-25 antibody;
The SNAP-25 snap25 (Catalog #MBS221415) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s SNAP-25 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistology Paraffin*, Western Blot (WB).Western Blot: This item detects a band of approximately 22kDa in human and mouse brain lysates. A minimum incubation time of 1 hour is recommended with this antibody.
ELISA: Maximum Dilution: 1/32000
Immunohistology - Paraffin: Minimum Dilution: 2.0; Maximum Dilution: 4.0ug/ml; Application Note: Heat-mediated antigen retrieval is required prior to staining paraffin sections with this antibody. MyBioSource recommend citrate buffer, pH6.0, for this purpose.
Western Blotting: Minimum Dilution: 0.01; Maximum Dilution: 0.03ug/ml. Researchers should empirically determine the suitability of the SNAP-25 snap25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SNAP-25 snap25 product has the following accession number(s) (GI #18765733) (NCBI Accession #NP_003072.2) (Uniprot Accession #P60880). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Goat ati Human SNAP25 antibody recognizes human synaptosomal-associated protein, 25kD (SNAP-25), also known as Super protein (Sup) or Synaptosomal-associated 25 kDa protein. SNAP-25 is a 206 amino acid, presynaptic plasma membrane protein containning 2 http://www.ebi.ac.uk/interpro/entry/IPR000727 domains and is essential for Ca2+-regulated exocytosis of neurotransmitters. SNAP-25 binds to and negatively modulate Ca2+ channels, increasing the speed of neurotransmitter release (Rettiq et al. 1996).Goat ati Human SNAP25 antibody detects an epitope within the C-terminal (CT) of human SNAP25 SNAP-25 is expressed by neurons and neuroendocrine cells as two predominant isoforms; SNAP-25a and SNAP-25b. SNAP-25a is expressed early in development, whereas SNAP-25b expression is linked to synaptogenesis. Goat ati human SNAP25 antibody is expected to recognise both isoforms. SNAP-25 may be a possible therapeutic treatment target for sufferers of hyperactivity disorders (Barr et al. 2000), whilst SNAP-25 based peptides may be a possible treatment for involuntary muscle spasms Hodel 1998)..
Perservative Stabilisers: 0.02% Sodium Azide
0.5% Bovine Serum Albumin. Buffer Solution: TRIS buffered saline
Antiserum Preparation: Antisera to human SNAP-25 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SNAP-25 are readily searchable from our website. Different antibodies against the same target such as SNAP-25 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.