anti-SPAK antibody product blog
Tags: Antibody; Polyclonal Antibody; spaK; anti-SPAK antibody;
The SPAK n/a (Catalog #MBS6004201) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPAK, Phosphorylated (Ser373), OSR1, Phosphorylated (Ser325) (STE20/SPS1-related Proline-alanine-rich Protein Kinase, Ste-20-related Kinase, DCHT, Serine/Threonine-protein Kinase 39, STK39/ Serine/Threonine-protein Kinase OSR1, Oxidative Stress-responsive reacts with Species Sequence Homology: Rat and canine, 100% and may cross-react with other species as described in the data sheet. MyBioSource\'s SPAK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Other applications not tested.Western Blot: 0.5 ug/mL
Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the SPAK n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SPAK n/a product has the following accession number(s) (GI #154687498) (NCBI Accession #YP_001422659.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SPAK, Phosphorylated (Ser373), OSR1, Phosphorylated (Ser325) (STE20/SPS1-related Proline-alanine-rich Protein Kinase, Ste-20-related Kinase, DCHT, Serine/Threonine-protein Kinase 39, STK39/ Serine/Threonine-protein Kinase OSR1, Oxidative Stress-responsive with the following immunoassay(s):
Chemiluminescence (Representative lot data. Untreated (lane 1) and lambda phosphatase- treated (lane 2), hypotonic-stimulated HEK293 cell lysates were probed with MBS6004201 (0.5ug/ml). Proteins were visualized using an IgG (HRP)donkey anti-rabbit Iand a chemiluminescence detection system. Arrow indicates SPAK (Ser373) / OSR1 (Ser325) (~60kD).)
As members of the (sterile 20)-like kinase family, the oxidative-stress-responsive kinase 1 (OSR1) and the SPS1-related proline/alanine-rich kinase (SPAK) serve as key enzymes in a signaling cascade, which mediate the activity of Na+/K+/2Cl- cotransporters (NKCCs) in response to osmotic stress. Both kinases have a conserved carboxy-terminal (CCT) domain, which are 79% identical to each other at the sequence level, and are also highly conserved in orthologues of these enzymes in Caenorhabditis elegans, Drosophila, and Xenopus. The CCT domain reflects no similarity to sequences found in other proteins. This domain is known to recognize and interact with an unique peptide (Arg-Phe-Xaa-Val) motif, which is present in OSRT1- and SPAK-activating kinases (with-no-lysine-kinase 1 (WNK1 and WNK4) and also in STE20 kinase family substrates (NKCC1 and NKCC2). It is reported that phosphorylation of a Ser/Thr residue preceding the (Arg-Phe-Xaa-Val) motif results in steric clash, and subsequent dissociation from the CCT domain. Furthermore, patients suffering from Gordon syndrome carry mutated WNK1 and WNK4 genes.