anti-SPARC antibody product blog
Tags: Antibody; Polyclonal Antibody; SPARC; anti-SPARC antibody;
The SPARC sparc (Catalog #MBS9412513) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPARC Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s SPARC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:100. Researchers should empirically determine the suitability of the SPARC sparc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SPARC sparc product has the following accession number(s) (GI #4507171) (NCBI Accession #NP_003109.1) (Uniprot Accession #P09486). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SPARC Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using SPARC antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat kidney using SPARC antibody at dilution of 1:100 (200x lens).)
SPARC (secreted protein acidic and rich in cysteine), also known as osteonectin and BM40, is a secreted matricellular glycoprotein that belongs to a group of functionally related glycoproteins that includes tenascins C and X, thrombospondins 1 and 2, and osteopontin (1). Members in this class of glycoproteins are involved in tissue renewal, tissue remodeling, and embryonic development and work by exerting counter-adhesive and antiproliferative effects that lead to changes in cell shape, disruption of cell adhesion, and inhibition of the cell cycle (2). SPARC is expressed at high levels in bone tissue but is widely distributed in many other tissues and cell types (3), and is known to be associated with tissues undergoing morphogenesis, angiogenesis, mineralization, and other pathological responses to injury and tumorigenesis (4,5). SPARC has also been linked with obesity and diabetes (6).
Immunogen Type: Recombinant Protein
Immunogen Description: Recombinant protein of human SPARC. Target Name: SPARC. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SPARC are readily searchable from our website. Different antibodies against the same target such as SPARC may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain Diseases, Breast Neoplasms, Disease Models, Animal, Fibrosis, Inflammation, Kidney Diseases, Lung Diseases, Lung Neoplasms, Necrosis, Neoplasms, Experimental are some of the diseases may be linked to SPARC Antibody.