anti-SPECC1 antibody product blog
Tags: Antibody; Polyclonal Antibody; SPECC1; anti-SPECC1 antibody;
The SPECC1 n/a (Catalog #MBS6001893) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPECC1, ID (SPECC1L, CYTSA, KIAA0376, Cytospin-A, Renal carcinoma antigen NY-REN-22, Sperm antigen with calponin homology and coiled-coil domains 1-like) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s SPECC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF).Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50
Immunofluorescence: 1:10-50. Researchers should empirically determine the suitability of the SPECC1 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SPECC1, ID (SPECC1L, CYTSA, KIAA0376, Cytospin-A, Renal carcinoma antigen NY-REN-22, Sperm antigen with calponin homology and coiled-coil domains 1-like) with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis in HL-60 cell line lysates (35ug/lane) using MBS6001893. This demonstrates that MBS6001893 detected the SPECC1 protein (arrow).)
Western Blot (WB) (Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) using MBS6001893. This demonstrates that MBS6001893 detected the SPECC1 protein (arrow).)
Testing Data
Flow Cytometry (FC/FACS) (Flow Cytometry analysis of HL-60 cells using MBS6001893 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of U-251MG cells using MBS6001893 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)
CYTSAis involved in cytokinesis and spindle organization.
Immunogen: SPECC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 712-741 amino acids from the Central region of human SPECC1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SPECC1 are readily searchable from our website. Different antibodies against the same target such as SPECC1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.