|The SPHK1 sphk1 (Catalog #MBS176029) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SPHK1 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s SPHK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC). Researchers should empirically determine the suitability of the SPHK1 sphk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SPHK1 sphk1 product has the following accession number(s) (GI #17369329) (NCBI Accession #Q9NYA1.1) (Uniprot Accession #Q9NYA1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-SPHK1 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-SPHK1 antibody, MBS176029, Western blotting
Lane 1: RAJI Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: Hut Cell Lysate
Lane 4: HELA Cell Lysate
Lane 5: 293T Cell Lysate
Immunohistochemistry (IHC) (Anti-SPHK1 antibody, MBS176029, IHC(P)
IHC(P): Human Placenta Tissue )
Immunocytochemistry (ICC) (Anti-SPHK1 antibody, MBS176029, ICC
ICC: HELA Cell )
Description: Rabbit IgG polyclonal antibody for Sphingosine kinase 1(SPHK1) detection. Tested with WB, IHC-P, ICC in Human, Mouse.
Background: SPHK1(Sphingosine Kinase 1), is an enzyme that in humans is encoded by the SPHK1 gene. Melendez et al.(2000) mapped the SPHK1 gene to chromosome 17q25.2 based on sequence identity with ESTs mapped to this region. Kohama et al.(1998) demonstrated that recombinant mouse Sphk1 can specifically phosphorylate D-erythro-sphingosine and that D, L-threo-dihydrosphingosine and N, N-dimethylsphingosine can act as competitive inhibitors of recombinant Sphk1. Pitson et al.(2000) found that recombinant SPHK1 and endogenous SPHK1 purified from placenta had identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties.
Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SPHK1 (117-133aa AASLNHYAGYEQVTNED), different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SPHK1 are readily searchable from our website. Different antibodies against the same target such as SPHK1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.