|The SPHK2 sphk2 (Catalog #MBS9208471) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPHK2 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s SPHK2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB).
WB~~1:1000. Researchers should empirically determine the suitability of the SPHK2 sphk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SPHK2 sphk2 product has the following accession number(s) (GI #323462183) (NCBI Accession #NP_001191087.1) (Uniprot Accession #Q9NRA0). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SPHK2 Antibody (C-term) with the following immunoassay(s):
Western Blot (WB) (SPHK2 Antibody (A605) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the SPHK2 antibody detected the SPHK2 protein (arrow).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Sphingosine Kinase (SphK) catalyzes the phosphorylation of the lipid sphingosine, creating the bioactive lipid sphingosine-1-phosphate (S1P). S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. SphK is an important signaling enzyme which is activated by diverse agents, including growth factors that signal through receptor tyrosine kinases, agents activating G protein-coupled receptors, and immunoglobulin receptors. Two SphK isotypes, SphK-1 and SphK-2, have been cloned, and both isotypes are ubiquitously expressed. SphK-1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. In contrast, SphK-2 increases apoptosis, and may be responsible for phosphorylating and activating the immunosuppressive drug FTY720.
Antigen Type: Synthetic Peptide. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SPHK2 are readily searchable from our website. Different antibodies against the same target such as SPHK2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.