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anti-SPIN2B antibody product blog

Posted on 2017-03-04 01:41:16 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; SPIN2B; anti-SPIN2B antibody;
The SPIN2B spin2b (Catalog #MBS2516928) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SPIN2B Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s SPIN2B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).
WB: 1:200-1:1000
IHC: 1:25-1:100. Researchers should empirically determine the suitability of the SPIN2B spin2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The SPIN2B spin2b product has the following accession number(s) (GI #544315537) (NCBI Accession #NP_001269390.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SPIN2B Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of SP20 cell using SPIN2B Polyclonal Antibody at dilution of 1:200)
Western Blot (WB) SPIN2B.

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded Human cervical cancer using SPIN2B Polyclonal Antibody at dilution of 1:25)
Immunohistochemistry (IHC) SPIN2B.

Human SPIN2 expressed by 2 IL3-dependent murine myeloid cell lines was moderately antiapoptotic following IL3 removal, but it was unable to block apoptosis induced by the chemotherapy agent doxorubicin. On the contrary, SPIN2-expressing cells were more sensitive to doxorubicin-mediated cell death. Also, SPIN2 did not combat the proapoptotic effects of Fas ligand stimulation in the Jurkat human T-cell line. Deletion of the C-terminal amino acids of SPIN2 diminished its antiapoptotic activity. In the murine myeloid cells, SPIN2 overexpression increased the cell number doubling times and slowed their rate of growth. There was also an increased percentage of cells in G2/M, which was more pronounced following IL3 withdrawal.

Immunogen: Recombinant protein of human SPIN2B
Calculated Molecular Weight: 29kDa. Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SPIN2B are readily searchable from our website. Different antibodies against the same target such as SPIN2B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Fatty Liver, Inflammation are some of the diseases may be linked to SPIN2B Polyclonal Antibody.
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