anti-sRANK antibody product blog
Tags: Antibody; RANKL; Polyclonal Antibody; sRANK; anti-sRANK antibody;
The sRANK tnfsf11 (Catalog #MBS695582) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Biotinylated Anti-hsRANKL reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s RANKL can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).Direct Detection: To detect hsRANKL by direct ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsRANKL.
Sandwich ELISA: To detect hsRANKL by sandwich ELISA (using 100 mul/well antibody solution) a concentration of 0.25 - 1.0 mug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human sRANKL as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsRANKL.
Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Researchers should empirically determine the suitability of the sRANK tnfsf11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The sRANK tnfsf11 product has the following accession number(s) (GI #12643360) (NCBI Accession #O14788.1) (Uniprot Accession #O14788). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hsRANKL. Anti-Human sRANKL specific antibody was purified by affinity chromatography and then biotinylated.
Immunogen: Recombinant Human sRANK Ligand. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing sRANK are readily searchable from our website. Different antibodies against the same target such as sRANK may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Alveolar Bone Loss, Breast Neoplasms, Cardiovascular Diseases, Inflammation, Necrosis, Neoplasm Metastasis, Osteolysis, Osteopetrosis, Osteoporosis, Pain are some of the diseases may be linked to Biotinylated Anti-hsRANKL. Brain, Connective Tissue, Liver, Lymph Node, Pancreas tissues are correlated with this protein. The following patways have been known to be associated with this gene.