anti-SUMO2 antibody product blog
Tags: Antibody; Polyclonal Antibody; SUMO2/3; SUMO2; anti-SUMO2 antibody;
The SUMO2 sumo2 (Catalog #MBS647322) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SUMO2/3, CT (SUMO3, SMT3B, SMT3H1, Small ubiquitin-related modifier 3, SMT3 homolog 1, SUMO-2, Ubiquitin-like protein SMT3B) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s SUMO2/3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100. Researchers should empirically determine the suitability of the SUMO2 sumo2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SUMO2 sumo2 product has the following accession number(s) (GI #54792071) (NCBI Accession #NP_001005849.1) (Uniprot Accession #P61956). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SUMO2/3, CT (SUMO3, SMT3B, SMT3H1, Small ubiquitin-related modifier 3, SMT3 homolog 1, SUMO-2, Ubiquitin-like protein SMT3B) with the following immunoassay(s):
Western Blot (WB) (The anti-SUMO2/3 C-term Pab is used in Western blot to detect SUMO2/3 in HeLa cell lysate.)
Western Blot (WB) (Western blot analysis of SUMO2/3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (Q65). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SUMO2/3 gene.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
SUMO2 and SUMO3 are members of the SUMO (small ubiquitin-like modifier) protein family. This protein family functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. In vertebrates, three members of the SUMO family have been described, SUMO 1 and the functionally distinct homologues SUMO 2 and SUMO 3. SUMO modification sites present in the N terminal regions of SUMO 2 and SUMO 3 are utilized by SAE1/SAE2 (SUMO E1) and Ubc9 (SUMO E2) to form polymeric chains of SUMO 2 and SUMO 3 on protein substrates, a property not shared by SUMO 1.
Immunogen: SUMO2/3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58~88 amino acids within C-terminal region of human SUMO2/3.