anti-TARDBP antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-TARDBP antibody; TARDBP;
The TARDBP tardbp (Catalog #MBS646168) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TARDBP, NT (TARDBP, TDP43, TAR DNA-binding protein 43) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s TARDBP can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the TARDBP tardbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TARDBP tardbp product has the following accession number(s) (GI #49065494) (NCBI Accession #CAG38565.1) (Uniprot Accession #Q13148). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the TARDBP, NT (TARDBP, TDP43, TAR DNA-binding protein 43) with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis in Hela cell line and mouse brain lysates (35ug/lane) using MBS646168. This demonstrates MBS646168 detected the TARDBP protein (arrow).)
Immunohistochemistry (IHC) (Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue using MBS646168 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates MBS646168 for immunohistochemistry.)
Immunofluorescence (IF) (Fluorescent confocal image of Hela cell stained with MBS646168. Hela cells were fixed with 4% PFA (20min), permeabilized with Triton X-100 (0.1%, 10min), then incubated with MBS646168 (1:25, 1hr at 37ºC). For secondary antibody, Alexa Fluor®488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50min at 37ºC). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1hr at 37ºC). Nuclei were counterstained with DAPI (blue) (10ug/ml, 10min). TARDBP immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.)
HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq].
Immunogen: TARDBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TARDBP. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TARDBP are readily searchable from our website. Different antibodies against the same target such as TARDBP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.