anti-TBX21 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-TBX21 antibody; TBX21; T-bet/Tbx21;
The TBX21 tbx21 (Catalog #MBS176063) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-T-bet/Tbx21 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s T-bet/Tbx21 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the TBX21 tbx21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The TBX21 tbx21 product has the following accession number(s) (GI #12230772) (NCBI Accession #Q9UL17.1) (Uniprot Accession #Q9UL17). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-T-bet/Tbx21 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-T-bet/Tbx21 antibody, MBS176063, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human TBX21,34.7KD(162aa tag+ V381-N535)
Lane 1: Recombinant Human TBX21 Protein 5ng
Lane 2: Recombinant Human TBX21 Protein 2.5ng
Lane 3: Recombinant Human TBX21 Protein 1.25ng )
Description: Rabbit IgG polyclonal antibody for T-box transcription factor TBX21(TBX21) detection. Tested with WB in Human, Mouse, Rat.
Background: TBX21(T-Box 21), also called TBET, is a protein that in humans is encoded by the TBX21 gene. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. Zhang and Yang(2000) mapped the mouse Tbx21 gene to chromosome 11D in an area showing homology of synteny with human chromosome 17. Szabo et al.(2000) showed that TBX21 expression correlates with IFNG expression in Th1 and natural killer(NK) cells. Ectopic expression of TBX21 both transactivated the IFNG gene and induced endogenous IFNG production. Finotto et al.(2002) observed reduced expression of the T(H)1 transcription factor Tbet in T cells from airways of patients with asthma compared with that in T cells from airways of nonasthmatic patients, suggesting that loss of Tbet might be associated with asthma.
Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human T-bet (522-535aa DKEAEGQFYNYFPN), different from the related rat and mouse sequences by one amino acid. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TBX21 are readily searchable from our website. Different antibodies against the same target such as TBX21 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.