anti-TDGF1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-TDGF1 antibody; TDGF1;
The TDGF1 tdgf1 (Catalog #MBS126607) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TDGF1 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s TDGF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the TDGF1 tdgf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TDGF1 tdgf1 product has the following accession number(s) (GI #117473) (NCBI Accession #P13385.1) (Uniprot Accession #P13385). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the TDGF1 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of HepG2 cells, using TDGF1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse kidney using TDGF1 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat kidney using TDGF1 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach using TDGF1 antibody at dilution of 1:100 (40x lens).)
Cripto, also known as teratocarcinoma derived growth factor 1 (TDGF-1), belongs to the EGF-CFC family of proteins. Members of this family are characterized by an N-terminal signal peptide, a conserved cysteine rich domain (CFC motif), and a short hydrophobic carboxy-terminal tail that contains GPI cleavage and attachment sites. The GPI moiety anchors Cripto and family members to the extracellular plasma membrane (1). An O-linked fucosylation site within the EGF-like motif is required for Cripto and related family members to perform their function as co-receptors for TGF-beta-related ligands such as Nodal and Vg1/GDF1 (2,3). Soluble forms of Cripto can be produced - these contain intact EGF and CFC domains, and are thought to have paracrine activities, as opposed to the autocrine activity of Cripto functioning as a coreceptor (4). Understanding of this paracrine activity is not complete, but it is proposed that Cripto may act as co-ligand for Nodal (3).Cripto is an important modulator of embryogenesis and oncogenesis (4). It is highly expressed in early embryos, and in embryonic stem (ES) cells where it is involved in cardiomyocytic differentiation and acts as a negative regulator of neurogenesis (5-7). Transient activation of Cripto is essential for the capacity of stem cell self-renewal and pluripotency in ES cells, and in some adult derived stem cells (8). Signaling through Cripto can also stimulate other activities that promote tumorigenesis such as stimulation of proliferation, cell motility, invasion, angiogenesis and epithelial-mesenchymal transition (EMT) (9-11). Cripto is highly expressed in a broad range of tumors, where it acts as a potent oncogene.