anti-TGF-alpha antibody product blog
Tags: Antibody; Polyclonal Antibody; TGF-alpha; anti-TGF-alpha antibody;
The TGF-alpha tgfa (Catalog #MBS691266) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Human TGF-alpha reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s TGF-alpha can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Neutr.Western Blot: To detect human TGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 g/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human TGF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Researchers should empirically determine the suitability of the TGF-alpha tgfa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TGF-alpha tgfa product has the following accession number(s) (GI #153791414) (NCBI Accession #NP_001093161.1) (Uniprot Accession #P01135). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Transforming Growth Factor-&alpha (TGF-alpha was originally isolated from the conditioned media of oncogenically-transformed cells as an EGF-like bioactivity. TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released from the transmembrane protein by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and seems to play a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. Mature TGF-alpha shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species-specific in its biological effects. TGF-alpha binds to the EGF receptor and activates the receptor tyrosine kinase. Accordingly, TGF-alpha shows a similar potency to EGF as a mitogen for fibroblasts and as an inducer of epithelial development in vivo. TGF-alpha is reportedly more potent than EGF as an angiogenic factor in vivo and as a stimulator for keratinocyte migration. The EGF receptor gene represents the cellular homologue of the avian v-erb-B oncogene.
Antibody Generation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant human TGF alpha (human Transforming Growth Factor-alpha). Anti-human TGF alpha specific antibody was purified by affinity chromatography employing immobilized human TGF alpha matrix. Buffer: PBS, pH 7.2
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of human TGF-alpha (2 ng/ml), a concentration of 0.1 - 0.15 g/ml of this antibody is required.