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anti-TIMP3 antibody product blog

Posted on 2017-06-29 16:22:07 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; TIMP3; anti-TIMP3 antibody;
The TIMP3 timp3 (Catalog #MBS126907) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TIMP3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s TIMP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the TIMP3 timp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The TIMP3 timp3 product has the following accession number(s) (GI #730948) (NCBI Accession #P35625.2) (Uniprot Accession #P35625). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the TIMP3 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TIMP3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB) TIMP3.

Immunofluorescence (IF) (Immunofluorescence analysis of MCF-7 cells using TIMP3 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF) TIMP3.

esultant inhibition of proteinase function (1,2). In addition to MMP inhibition, TIMPs have also been shown to interact with various membrane receptors on the cell surface. Some of these interactions include: TIMP1 with CD63, TIMP2 with alpha3beta1 integrin, and TIMP3 with VEGFR2, all of which result in distinct cellular effects (3). TIMPs are involved in a wide variety of biological functions, such as tumor angiogenesis and progression (4,5), wound healing, and vascular remodeling (6,7). Mutations in TIMP3 are associated with Sorsby\'s fundus dystrophy (8,9).

Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human TIMP3
Calculated Molecular Weight: 24kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TIMP3 are readily searchable from our website. Different antibodies against the same target such as TIMP3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. TIMP3 also interacts with the following gene(s): ADAM17, EFEMP1, GSTP1, MMP2, MMP3, MMP9, OSM, TGFB1, TP53. Blood, Bone, Brain, Embryonic Tissue, Eye, Heart, Liver, Lung, Muscle, Vascular tissues are correlated with this protein.
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