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anti-TLR8 antibody product blog

Posted on 2015-10-12 14:27:36 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; TLR8; anti-TLR8 antibody;
The TLR8 tlr8 (Catalog #MBS127119) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TLR8 Polyclonal Antibody reacts with Human, Mouse. and may cross-react with other species as described in the data sheet. MyBioSource\'s TLR8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF).
WB: 1:500 - 1:2000
IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the TLR8 tlr8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The TLR8 tlr8 product has the following accession number(s) (GI #20140873) (NCBI Accession #Q9NR97.1) (Uniprot Accession #Q9NR97). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the TLR8 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of mouse liver, using TLR8 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.)
Western Blot (WB) TLR8.

The Toll-like Receptors (TLR) are a family of human receptors that share homology with the Drosophila Toll Receptors, which are involved in mediating dorsoventral polarization in developing Drosophila embryos and participate in host immunity. The TLR family members are characterized by a highly conserved Toll homology (TH) domain, which is essential for Toll-induced signal transductions. TLRs are type I transmembrane receptors that contain an extracellular domain consisting of several leucine-rich regions and a single cytoplasmic Toll/IL-1R like domain. Three TLR family members, TLR7, TLR8 and TLR9, belong to a subfamily of TLRs which are differentially expressed. TLR7 is expressed in lung, placenta and spleen. TLR8 is expressed in lung and peripheral blood leukocytes, and TLR9 is predominantly expressed in spleen, lymph nodes, bone marrow and peripheral blood leukocytes. TLR7, TLR8 and TLR9 stimulate the NF˚B signaling pathway, suggesting that they play a role in the immune response.

Source: Rabbit. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Immunogen: Recombinant protein of human TLR8. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TLR8 are readily searchable from our website. Different antibodies against the same target such as TLR8 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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