|The TMED10 tmed10 (Catalog #MBS150954) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TMP21 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s TMP21 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
TMP21 antibody can be used for detection of TMP21 by Western blot at 0.5 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the TMED10 tmed10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TMED10 tmed10 product has the following accession number(s) (GI #4885697) (NCBI Accession #AAD31941) (Uniprot Accession #P49755). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the TMP21 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of TMP21 in Raji cell lysate with TMP21 antibody at (A) 0.5 and (B) 1 and (C) 2 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of TMP21 in human brain tissue with TMP21 antibody at 2.5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of TMP21 in Human Brain cells with TMP21 antibody at 20 μg/mL.)
TMP21 Antibody: TMP21 is an ubiquitously expressed protein that is involved in vesicular targeting and protein transport. More recent experiments have shown that it is also a component in the presenilin complex and modulates the gamma-secretase but not the epsilon-secretase cleavage activity of the amyloid precursor protein. The presenilin complex is composed of the proteins APH1, nicastrin, and PEN2 in addition to presenilin-1. Together, these proteins cleave the amyloid precursor protein at what is known as the gamma- and epsilon-sites and can lead to the accumulation of the Abeta cleavage product that is associated with Alzheimer\'s disease. Co-immunoprecipitation experiments using antibodies against these proteins also yielded TMP21 indicating that TMP21 may play a role in the regulation of this complex. Suppression of TMP21 expression by siRNA in transfected cells caused increased gamma-secretase activity but not epsilon-secretase activity, and increased Abeta production, demonstrating that TMP21 can modulate gamma-secretase activity.
Immunogen: TMP21 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human TMP21.
Buffer: TMP21 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TMED10 are readily searchable from our website. Different antibodies against the same target such as TMED10 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.