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anti-TRIM5 antibody product blog

Posted on 2015-07-22 09:20:31 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; anti-TRIM5 antibody; TRIM5; TRIM5 alpha;
The TRIM5 trim5 (Catalog #MBS150100) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM5 alpha Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s TRIM5 alpha can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
TRIM5 alpha antibody can be used for detection of TRIM5 alpha by Western blot at 2 mug/mL. A band at approximately 55 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 mug/mL. For immunofluorescence start at 10 mug/mL. Researchers should empirically determine the suitability of the TRIM5 trim5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The TRIM5 trim5 product has the following accession number(s) (GI #14719418) (NCBI Accession #NP_149023) (Uniprot Accession #Q9C035). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the TRIM5 alpha Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of TRIM5 alpha expression in human stomach (A), thymus (B), and uterus (C) cell lysate with TRIM5 alpha antibody at 2 μg /ml.)
Western Blot (WB) TRIM5.

Immunohistochemistry (IHC) (Immunohistochemical staining of human stomach using TRIM5 alpha antibody at 2 μg/mL.)
Immunohistochemistry (IHC) TRIM5.

Immunofluorescence (IF) (Immunofluorescence of TRIM5 alpha in Human Stomach cells with TRIM5 alpha antibody at 10 μg/mL.)
Immunofluorescence (IF) TRIM5.

TRIM5 alpha Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has six alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. All TRIM5α variants could inhibit at least two different retroviruses, but not from those viruses isolated from the same species, suggesting that TRIM5α acts as a natural barrier to cross-species retrovirus transmission.

Conjugate: Unconjugated
Immunogen: TRIM5 alpha antibody was raised against a synthetic peptide corresponding to amino acids near the mid-region of human TRIM5 alpha.
Buffer: TRIM5 alpha Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TRIM5 are readily searchable from our website. Different antibodies against the same target such as TRIM5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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