anti-TRIM5 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-TRIM5 antibody; TRIM5; TRIM5 delta;
The TRIM5 trim5 (Catalog #MBS151206) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM5 delta Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s TRIM5 delta can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).TRIM5 delta antibody can be used for detection of TRIM5 delta by Western blot at 2 mug/mL. A band at approximately 25 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 1 mug/mL. For immunofluorescence start at 10 mug/mL. Researchers should empirically determine the suitability of the TRIM5 trim5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The TRIM5 trim5 product has the following accession number(s) (GI #15011946) (NCBI Accession #NP_149084) (Uniprot Accession #Q9C035). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the TRIM5 delta Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of TRIM5 delta in human kidney tissue lysate with TRIM5 delta antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC) (Immunohistochemistry of TRIM5 delta in mouse brain tissue with TRIM5 delta antibody at 1 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of TRIM5 delta in mouse brain tissue with TRIM5 delta antibody at 10 μg/mL.)
TRIM5 delta Antibody: TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has five alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5α variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1, albeit at differing efficiencies. The TRIM5δ isoform has been shown to colocalize with the topoisomerase I-interacting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5δ also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.
Conjugate: Unconjugated
Immunogen: TRIM5 delta antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy terminus of human TRIM5 delta.
Buffer: TRIM5 delta Antibody is supplied in PBS containing 0.02% sodium azide.