|The TSHZ2 n/a (Catalog #MBS248786) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human TSHZ2 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s TSHZ2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS).
Flo (1:10 - 1:50), IF (1:10 - 1:50), IHC-P (5 ug/ml), WB (1:1000). Researchers should empirically determine the suitability of the TSHZ2 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Polyclonal to Human TSHZ2 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-TSHZ2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
Immunohistochemistry (IHC) (TSH2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This demonstrates the use of the TSH2 Antibody (N-term) for immunohistochemistry.)
Immunofluorescence (IF) (Fluorescent confocal image of U251 cell stained with TSH2 Antibody (N-term).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSH2 primary antibody (1:25, 1 h at 37[Character 2103]). For secondary antibody, Alexa Fluor[Character ae] 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37[Character 2103]).Cytoplasmic actin was counterstained with Alexa Fluor[Character ae] 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37[Character 2103]). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TSH2 immunoreactivity is localized to Nucleus and Vesicles significantly.)
Flow Cytometry (FC/FACS) (TSH2 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blot analysis of TSH2 Antibody (N-term) in CEM cell line lysates (35 ug/lane).TSH2 (arrow) was detected using the purified Pab.)
Target Species: Human
Immunogen Description: KLH-conjugated synthetic peptide from N-terminal region of human TSH2. Immunogen Type: Synthetic peptide
Immunogen: TSHZ2 antibody was raised against kLH-conjugated synthetic peptide from N-terminal region of human TSH2.
Antigen Modification: aa470-500. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing TSHZ2 are readily searchable from our website. Different antibodies against the same target such as TSHZ2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.