anti-UBB antibody product blog
Tags: Antibody; Ubiquitin; Polyclonal Antibody; anti-UBB antibody; UBB;
The UBB ubb (Catalog #MBS806362) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ubiquitin Antibody: PerCP reacts with Human, Monkey, Mouse, Rat, Hamster, Rabbit, Guinea pig, Bovine, Pig, Canine, Sheep, Chicken, Xenopus, Yeast, Drosophila, Fish (Rainbow trout) and may cross-react with other species as described in the data sheet. MyBioSource\'s Ubiquitin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), chIP, Immunofluorescence (IF).1:1000-1500 (ECL)(WB). Researchers should empirically determine the suitability of the UBB ubb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The UBB ubb product has the following accession number(s) (GI #27806505) (NCBI Accession #NP_776558.1) (Uniprot Accession #P0CG53). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Ubiquitin Antibody: PerCP with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293) lysate showing detection of Ubiquitin protein using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:1000.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: backskin. Species: Mouse. Fixation: Bouin\'s Fixative Solution. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasm.)
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Rabbit Anti-Ubiquitin Polyclonal Antibody (SPC-119) at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT.)
Background Info: The antibody recognizes ~10kDa corresponding to free ubiquitin of human, mouse, monkey, bovine and rat origins. It also recognizes ubiquinated proteins.
Scientific Background: Ubiquitin is a small protein that occurs in all eukaryotic Ubiquitin is a small protein that occurs in all eukaryotic.
Conjugate: PerCP. Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing UBB are readily searchable from our website. Different antibodies against the same target such as UBB may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.