anti-UBB antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-UBB antibody; UBB; UBB (Ubiquitin);
The UBB ubb (Catalog #MBS9213906) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBB (Ubiquitin) Antibody (N-term) reacts with Human, mouse, rat (Predicted Reactivity: Bovine, C Elegans, Drosophila, Monkey, Pig, Chicken, Hamster, Xenopus, Rabbit) and may cross-react with other species as described in the data sheet. MyBioSource\'s UBB (Ubiquitin) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).WB~~1:1000. Researchers should empirically determine the suitability of the UBB ubb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The UBB ubb product has the following accession number(s) (GI #528524471) (NCBI Accession #NP_001268645.1) (Uniprot Accession #P0CG47). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the UBB (Ubiquitin) Antibody (N-term) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of lysates from Hela, HepG2, Jurkat cell line, mouse liver, rat kidney tissue(from left to right), using UBB(Ubiquitin) Antibody (N-term). MBS9213906 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Crown Antibody: Yes. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing UBB are readily searchable from our website. Different antibodies against the same target such as UBB may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.