anti-UBD antibody product blog
Tags: Antibody; Polyclonal Antibody; uBD; FAT10; anti-UBD antibody;
The UBD ubd (Catalog #MBS9204261) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FAT10 Antibody (C-term) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s FAT10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunofluorescence (IF).WB~~1:1000
IHC-P~~1:50~100
IF~~1:10~50. Researchers should empirically determine the suitability of the UBD ubd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The UBD ubd product has the following accession number(s) (GI #222352096) (NCBI Accession #NP_006389.2) (Uniprot Accession #O15205). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the FAT10 Antibody (C-term) with the following immunoassay(s):
Western Blot (WB) (FAT10 Antibody (C-term) (MBS9204261) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the hFAT10 antibody detected the hFAT10 protein (arrow).)
Immunohistochemistry (IHC) (FAT10 Antibody (C-term) (MBS9204261) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of FAT10 Antibody (C-term) (MBS9204261) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of FAT10 Antibody (C-term) (MBS9204261) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Function: Ubiquitin-like protein modifier which can becovalently attached to target protein and subsequently leads to their degradation bythe 26S proteasome, in a NUB1-dependentmanner. Probably functions as a survival factor. Conjugation ability activated by UBA6. Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediatedactivation of the central mediator of innateimmunity NF-kappa-B by promotingTNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha. Required forTNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). Maybe involved in dendritic cell (DC) maturation,the process by which immature dendritic cellsdifferentiate into fully competentantigen-presenting cells that initiate T-cellresponses. Mediates mitotic non-disjunctionand chromosome instability, in long- term invitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle. May be involved in the formation of aggresomeswhen proteasome is saturated or impaired. Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium andtubular cells during renal diseases such aspolycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN). Cellular Location: Nucleus. Cytoplasm Note=Accumulates in aggresomes under proteasome inhibition conditions
Tissue Location: Constitutively expressed in mature dendritic cells and B-cells. Mostly expressed in the reticuloendothelial system (e.g. thymus, spleen), the gastrointestinal system, kidney, lung and prostate gland.