anti-UBE2L3 antibody product blog
Tags: Antibody; Polyclonal Antibody; UBE2L3; anti-UBE2L3 antibody;
The UBE2L3 ube2l3 (Catalog #MBS129127) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UBE2L3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s UBE2L3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).WB: 1:1000 - 1:2000. Researchers should empirically determine the suitability of the UBE2L3 ube2l3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The UBE2L3 ube2l3 product has the following accession number(s) (GI #54039805) (NCBI Accession #P68036.1) (Uniprot Accession #P68036). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the UBE2L3 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using UBE2L3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat heart using UBE2L3 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach using UBE2L3 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse brain using UBE2L3 antibody at dilution of 1:100 (40x lens).)
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human UBE2L3
Calculated Molecular Weight: 18kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing UBE2L3 are readily searchable from our website. Different antibodies against the same target such as UBE2L3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.