anti-UVRAG antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-UVRAG antibody; UVRAG;
The UVRAG uvrag (Catalog #MBS6001910) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The UVRAG, CT (UV Radiation Resistance-associated Gene Protein, p63, DHTX, VPS38) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s UVRAG can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC).Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunoprecipitation: 5ug/ml
Immunohistochemistry: 1:50-100
immunofluorescence tested. Researchers should empirically determine the suitability of the UVRAG uvrag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The UVRAG uvrag product has the following accession number(s) (GI #21687212) (NCBI Accession #NP_003360.2) (Uniprot Accession #Q9P2Y5). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the UVRAG, CT (UV Radiation Resistance-associated Gene Protein, p63, DHTX, VPS38) with the following immunoassay(s):
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with UVRAG (C-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1850b UVRAG (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). UVRAG immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)
Western Blot (WB) (Immunoprecipitation and western blot analysis of anti-UVRAG Pab in 293T cells. UVRAG is immunoprecipitated (Lane 2) and detected in 293T cell transiently transfected with mouse UVRAG (Lane 1). Detection of endogenous UVRAG is shown in 293T cells (Lane 3) but is reduced by UVRAG siRNA transfection (Lane 4). Data courtesy of Dr. Hong-Gang Wang, Moffitt Cancer Center, Tampa, FL.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with hUVRAG (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunofluorescence (IF) (Immunofluorescence staining of Autophagy UVRAG antibody on Methanol-fixed HeLa cells. Data courtesy of Dr. Eeva-Liisa Eskelinen, University of Helsinki,Finland.)
UVRAG complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppressing the proliferation and tumorigenicity of human colon cancer cells. Chromosomal aberrations involving this gene are associated with left-right axis malformation and mutations in this gene have been associated with colon cancer.