anti-VASP antibody product blog
Tags: Antibody; Polyclonal Antibody; VASP; anti-VASP antibody; Vasodilator Stimulated Phosphoprotein;
The VASP vasp (Catalog #MBS616533) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Vasodilator Stimulated Phosphoprotein (VASP) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Vasodilator Stimulated Phosphoprotein can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the VASP vasp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The VASP vasp product has the following accession number(s) (GI #4507869) (NCBI Accession #NP_003361.1) (Uniprot Accession #P50552). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Vasodilator Stimulated Phosphoprotein (VASP) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from A431 cells stimulated with forskolin for indicated times, using VASP Antibody.)
Testing Data
Vasodilator-stimulated phosphoprotein (VASP) was originally characterized as a substrate of both cGMP-and cAMP-dependent kinases (PKG and PKA, or cGPK and cAPK, respectively) (1). It is now believed that VASP belongs to the Ena/VASP family of adaptor proteins linking the cytoskeletal system to the signal transduction pathways and functions in cytoskeletal organization, fibroblast migration, platelet activation and axon guidance (2,3). Three phosphorylation sites, Ser157, Ser239 and Thr278, have been identified. Ser239 is the major PKG phosphorylation site while Ser157 is the major PKA phosphorylation site (4). Evidence suggests that VASP phosphorylation reduces its association with actin and has a negative effect on actin polymerization (5). Phosphorylation at Ser239 of VASP is an useful tool to monitor PKG activation and signaling (6,7).
Immunogen: Synthetic peptide (KLH coupled) corresponding to residues surrounding Ser157 of human VASP. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing VASP are readily searchable from our website. Different antibodies against the same target such as VASP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Acute Coronary Syndrome, Arteriosclerosis, Cardiovascular Diseases, Coronary Disease, Inflammation, Metabolic Diseases, Myocardial Infarction, Myocardial Ischemia, Pain, Thrombosis are some of the diseases may be linked to Vasodilator Stimulated Phosphoprotein (VASP).