anti-VAV3 antibody product blog
Tags: Antibody; Polyclonal Antibody; VAV3; anti-VAV3 antibody;
The VAV3 vav3 (Catalog #MBS7051579) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VAV3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s VAV3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).WB: 1: 500-1: 5000
IHC: 1: 20-1: 200. Researchers should empirically determine the suitability of the VAV3 vav3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The VAV3 vav3 product has the following accession number(s) (GI #120432042) (NCBI Accession #NP_001073343.1) (Uniprot Accession #Q9UKW4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the VAV3 Antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot
Positive WB detected in: Mouse kidney tissue,Rat lung tissue
All lanes: VAV3 antibody at 2.5ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 98,87,33,101 KDa
Observed band size: 98 KDa
)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human pancreatic tissue using MBS7051579 at dilution of 1:100)
Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2 (ITGB2)-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3 (ITGB3)-mediated adhesion. Does not affect integrin beta-1 (ITGB1)-mediated adhesion (By similarity).
Immunogen: Recombinant Human Guanine nucleotide exchange factor VAV3 protein (562-674AA). Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Conjugation: Non-conjugated
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-28474 / sc-28474. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing VAV3 are readily searchable from our website. Different antibodies against the same target such as VAV3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.