|The VCL vcl (Catalog #MBS621491) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Vinculin, phosphorylated (Tyr822) reacts with Chicken, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Vinculin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the VCL vcl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The VCL vcl product has the following accession number(s) (GI #246714) (Uniprot Accession #P18206). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Vinculin is an ubiquitously expressed cytoskeletal protein (~130kD) involved in cell adhesion and cell migration. The vinculin protein consists of a globular head domain connected to an elongated tail region by a proline-rich domain. The head region contains binding sites for two cytoskeletal proteins, a-actinin and talin, as well as a binding site for the tail region of vinculin itself. The linker region possesses binding sites for two adaptor proteins, vinexin and ponsin, that may connect growth factor receptors with integrin signaling pathways, and a binding site for an actin-bundling protein, VASP. The tail region contains binding sites for actin, the cytoskeletal protein, paxillin, and PI(4,5)P2. In the inactive state the head region of vinculin is bound to the tail region, resulting in inaccessibility of the other protein binding sites. Binding of PI(4,5)P2 releases the head-tail interaction allowing binding of other proteins to vinculin. These regulatory events play an important role in the formation, maintenance, and breakdown of focal adhesions that occur during cell adhesion and migration. In addition to these protein binding sites, the head and tail regions of vinculin have multiple potential phosphorylation sites. Data indicate the possible involvement of phosphorylation of tyrosine 822 of vinculin with the integrity/strength of integrinmediated focal adhesions.
Immunogen: Synthetic phosphopeptide derived from a region of human vinculin that contains tyrosine 822. Positive Control: Chick Embryo Fibroblasts (CEFs) transfected with activated Src. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing VCL are readily searchable from our website. Different antibodies against the same target such as VCL may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.