|The VDR vdr (Catalog #MBS127530) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VDR Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s VDR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the VDR vdr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The VDR vdr product has the following accession number(s) (GI #137617) (NCBI Accession #P11473.1) (Uniprot Accession #P11473). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the VDR Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of mouse kidney, using VDR antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
The vitamin D receptor (VDR), also known as the calcitriol receptor, and also known as NR1I1 (nuclear receptor subfamily 1, group I, member 1), is a member of the nuclear receptor family of transcription factors. Upon activation by vitamin D, the VDR forms a heterodimer with the retinoid-X receptor and binds to hormone response elements on DNA resulting in expression or trans-repression of specific gene products.It is an intracellular hormone receptor that specifically binds 1,25(OH)2D3 and mediates its effects. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A). A disorder of vitamin D metabolism results in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. This antibody is a rabbit Primary antibodyto human VDR.
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human VDR
Calculated Molecular Weight: 48kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing VDR are readily searchable from our website. Different antibodies against the same target such as VDR may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.