anti-VGLU2 antibody product blog
Tags: Antibody; Polyclonal Antibody; VGLU2; anti-VGLU2 antibody;
The VGLU2 n/a (Catalog #MBS6004735) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The VGLU2, CT (Slc17a6, Dnpi, Vglut2, Vesicular glutamate transporter 2, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s VGLU2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:50-100. Researchers should empirically determine the suitability of the VGLU2 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the VGLU2, CT (Slc17a6, Dnpi, Vglut2, Vesicular glutamate transporter 2, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 6) with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis in mouse cerebellum, brain tissue lysates (35ug/lane) using MBS6004735. VGLU2 (arrow) was detected using MBS6004735.)
Testing Data (All lanes: MBS6004735 (1:1000-1:2000) Lane 1: Human brain lysate Lane 2: Human fetal brain lysate Lane 3: Human cerebellum lysate Lane 4: Mouse cerebellum lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 65kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (All lanes: MBS6004735 (1:2000) Lane 1: Human fetal brain lysate Lane 2: Human cerebellum lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 65kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain reacted with MBS6004735, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6004735 for immunohistochemistry; clinical relevance has not been evaluated.)
MOUSE VGLU2 mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. This protein may also mediate the transport of inorganic phosphate.
Immunogen: MOUSE VGLU2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 514~543 amino acids from the C-terminal region of MOUSE VGLU2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing VGLU2 are readily searchable from our website. Different antibodies against the same target such as VGLU2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.